Silbiger Lab General Protocols

General protocols and manuals used by the Silbiger Lab for various research projects and equipment

Physiological Protocols

• Ash Free Dry Weight
  • Box Furnace Procedure
• Chlorophyll Extraction
• Symbiont Cell Counts
• Tissue Removal
• Tissue N Content
• Respirometry protocol

Environmental Parameter Protocols

• Nutrients in situ
• Macroalgal CHN Content
• Sedimentation Rates
• Volume Dye Method Protocols
• RAD7 Protocols
• RAD Float Setup
• Light Spectrum Protocols
• Sub-surface Automated Sampler Protocols

Intertidal Ecosystem Function Protocols

• Tide Pool Water Sampling

Probe and Logger Protocols

• YSI 2030 Calibration
• Orion Calibration
• HOBO Conductivity and Temperature Logger
• HOBO Depth/Pressure Logger
• HOBO pH Logger
• HOBO CT, Water Level, and pH Data Processing Dashboard (ShinyApp)
• LI-COR PAR Sensor
• miniDOT Oxygen Sensor
• SLED Deployment and Collection

Titration Protocols

Titration Repository

• Titrator SOP
• Automatically transfer weights from mettler balance to titrator or excel file
• Acid washing bottles
• Mixing Mercuric Chloride solution for poisoning water samples
• Ordering and returning carbonate chemistry solutions to Dickson Lab

Spectrophotometer Protocols

• m-Cresol Purple Protocol

SpectraMax iD3 Repository

• Startup Procedures
• Plate Read Setup
• Preparing the Well Plate
• Export Data

UV 2450 Spectrophotometer Protocol (Mo'orea and Polyp Lab)

Mo'orea Guides

Private Repository for navigating Mo'orea travel

Student Resources

Private Repository with guides and information for students navigating a CSUN Biology Masters degree program

Reference Manuals

• Box Furnace
• RAD7 and RAD aqua
• Respirometry
• LI-COR PAR Sensor
• miniDOT Oxygen Sensor
• HOBO Conductivity and Temperature Logger
• HOBO Pressure/Depth Logger
• HOBO pH Logger
• SpectraMax iD3
• Spectronic Genesys 5
• Smart Spec 3000