diff --git a/.conda/meta.yaml b/.conda/meta.yaml
index 2f433a28e..30fc67306 100644
--- a/.conda/meta.yaml
+++ b/.conda/meta.yaml
@@ -16,12 +16,12 @@ build:
 
 requirements:
   host:
-    - python >=3.10
+    - python >=3.11
     - hatchling
     - hatch-vcs
 
   run:
-    - python >=3.10
+    - python >=3.11
     - anndata >=0.9
     - awkward >=2.1.0
     - mudata >=0.2.3
diff --git a/.cruft.json b/.cruft.json
index dfb7cf65f..d73a98cd2 100644
--- a/.cruft.json
+++ b/.cruft.json
@@ -1,43 +1,43 @@
 {
-    "template": "https://github.com/scverse/cookiecutter-scverse",
-    "commit": "d383d94fadff9e4e6fdb59d77c68cb900d7cedec",
-    "checkout": "v0.6.0",
-    "context": {
-        "cookiecutter": {
-            "project_name": "scirpy",
-            "package_name": "scirpy",
-            "project_description": "A very interesting piece of code",
-            "author_full_name": "Gregor Sturm",
-            "author_email": "mail@gregor-sturm.de",
-            "github_user": "grst",
-            "github_repo": "scirpy",
-            "license": "BSD 3-Clause License",
-            "ide_integration": true,
-            "_copy_without_render": [
-                ".github/workflows/build.yaml",
-                ".github/workflows/test.yaml",
-                "docs/_templates/autosummary/**.rst"
-            ],
-            "_exclude_on_template_update": [
-                "CHANGELOG.md",
-                "LICENSE",
-                "README.md",
-                "docs/api.md",
-                "docs/index.md",
-                "docs/notebooks/example.ipynb",
-                "docs/references.bib",
-                "docs/references.md",
-                "src/**",
-                "tests/**"
-            ],
-            "_render_devdocs": false,
-            "_jinja2_env_vars": {
-                "lstrip_blocks": true,
-                "trim_blocks": true
-            },
-            "_template": "https://github.com/scverse/cookiecutter-scverse",
-            "_commit": "d383d94fadff9e4e6fdb59d77c68cb900d7cedec"
-        }
-    },
-    "directory": null
+  "template": "https://github.com/scverse/cookiecutter-scverse",
+  "commit": "6ff5b92b5d44ea6d8a88e47538475718d467db95",
+  "checkout": "v0.7.0",
+  "context": {
+    "cookiecutter": {
+      "project_name": "scirpy",
+      "package_name": "scirpy",
+      "project_description": "A very interesting piece of code",
+      "author_full_name": "Gregor Sturm",
+      "author_email": "mail@gregor-sturm.de",
+      "github_user": "grst",
+      "github_repo": "scirpy",
+      "license": "BSD 3-Clause License",
+      "ide_integration": true,
+      "_copy_without_render": [
+        ".github/workflows/build.yaml",
+        ".github/workflows/test.yaml",
+        "docs/_templates/autosummary/**.rst"
+      ],
+      "_exclude_on_template_update": [
+        "CHANGELOG.md",
+        "LICENSE",
+        "README.md",
+        "docs/api.md",
+        "docs/index.md",
+        "docs/notebooks/example.ipynb",
+        "docs/references.bib",
+        "docs/references.md",
+        "src/**",
+        "tests/**"
+      ],
+      "_render_devdocs": false,
+      "_jinja2_env_vars": {
+        "lstrip_blocks": true,
+        "trim_blocks": true
+      },
+      "_template": "https://github.com/scverse/cookiecutter-scverse",
+      "_commit": "6ff5b92b5d44ea6d8a88e47538475718d467db95"
+    }
+  },
+  "directory": null
 }
diff --git a/.github/ISSUE_TEMPLATE/bug_report.yml b/.github/ISSUE_TEMPLATE/bug_report.yml
index 3ca1ccbde..6104b9e6f 100644
--- a/.github/ISSUE_TEMPLATE/bug_report.yml
+++ b/.github/ISSUE_TEMPLATE/bug_report.yml
@@ -1,6 +1,6 @@
 name: Bug report
 description: Report something that is broken or incorrect
-labels: bug
+type: Bug
 body:
   - type: markdown
     attributes:
@@ -9,8 +9,7 @@ body:
         detailing how to provide the necessary information for us to reproduce your bug. In brief:
           * Please provide exact steps how to reproduce the bug in a clean Python environment.
           * In case it's not clear what's causing this bug, please provide the data or the data generation procedure.
-          * Sometimes it is not possible to share the data, but usually it is possible to replicate problems on publicly
-            available datasets or to share a subset of your data.
+          * Replicate problems on public datasets or share data subsets when full sharing isn't possible.
 
   - type: textarea
     id: report
diff --git a/.github/ISSUE_TEMPLATE/feature_request.yml b/.github/ISSUE_TEMPLATE/feature_request.yml
index 585af6936..a1f032979 100644
--- a/.github/ISSUE_TEMPLATE/feature_request.yml
+++ b/.github/ISSUE_TEMPLATE/feature_request.yml
@@ -1,6 +1,6 @@
 name: Feature request
 description: Propose a new feature for scirpy
-labels: enhancement
+type: Enhancement
 body:
   - type: textarea
     id: description
diff --git a/.github/workflows/build.yaml b/.github/workflows/build.yaml
index 83e01a1ee..c6ecc2f25 100644
--- a/.github/workflows/build.yaml
+++ b/.github/workflows/build.yaml
@@ -10,23 +10,16 @@ concurrency:
   group: ${{ github.workflow }}-${{ github.ref }}
   cancel-in-progress: true
 
-defaults:
-  run:
-    # to fail on error in multiline statements (-e), in pipes (-o pipefail), and on unset variables (-u).
-    shell: bash -euo pipefail {0}
-
 jobs:
   package:
     runs-on: ubuntu-latest
     steps:
-      - uses: actions/checkout@v4
+      - uses: actions/checkout@v5
         with:
           filter: blob:none
           fetch-depth: 0
       - name: Install uv
-        uses: astral-sh/setup-uv@v5
-        with:
-          cache-dependency-glob: pyproject.toml
+        uses: astral-sh/setup-uv@v7
       - name: Build package
         run: uv build
       - name: Check package
diff --git a/.github/workflows/release.yaml b/.github/workflows/release.yaml
index d4f2594aa..b23933593 100644
--- a/.github/workflows/release.yaml
+++ b/.github/workflows/release.yaml
@@ -4,11 +4,6 @@ on:
   release:
     types: [published]
 
-defaults:
-  run:
-    # to fail on error in multiline statements (-e), in pipes (-o pipefail), and on unset variables (-u).
-    shell: bash -euo pipefail {0}
-
 # Use "trusted publishing", see https://docs.pypi.org/trusted-publishers/
 jobs:
   release:
@@ -20,14 +15,12 @@ jobs:
     permissions:
       id-token: write # IMPORTANT: this permission is mandatory for trusted publishing
     steps:
-      - uses: actions/checkout@v4
+      - uses: actions/checkout@v5
         with:
           filter: blob:none
           fetch-depth: 0
       - name: Install uv
-        uses: astral-sh/setup-uv@v5
-        with:
-          cache-dependency-glob: pyproject.toml
+        uses: astral-sh/setup-uv@v7
       - name: Build package
         run: uv build
       - name: Publish package distributions to PyPI
diff --git a/.github/workflows/test-tutorials.yaml b/.github/workflows/test-tutorials.yaml
index fa3848c3f..c2f9439bb 100644
--- a/.github/workflows/test-tutorials.yaml
+++ b/.github/workflows/test-tutorials.yaml
@@ -26,32 +26,23 @@ jobs:
           - tutorial_5k_bcr.ipynb
         os:
           - ubuntu-latest
-        python:
-          - "3.10"
-
-    env:
-      OS: ${{ matrix.os }}
-      PYTHON: ${{ matrix.python }}
 
     steps:
-      - uses: actions/checkout@v3
-      - name: Set up Python ${{ matrix.python }}
-        uses: actions/setup-python@v4
+      - uses: actions/checkout@v4
         with:
-          python-version: ${{ matrix.python }}
-          cache: "pip"
-          cache-dependency-path: "**/pyproject.toml"
-
+          filter: blob:none
+          fetch-depth: 0
       - name: Install system dependencies
         run: |
           sudo apt-get update && sudo apt-get install -y libcairo2-dev pkg-config
-      - name: Install dependencies
-        run: |
-          pip install ".[doc]"
+      - name: Install uv
+        uses: astral-sh/setup-uv@v7
+        with:
+          cache-dependency-glob: pyproject.toml
       - name: Test
         env:
           MPLBACKEND: agg
           PLATFORM: ${{ matrix.os }}
           DISPLAY: :42
         run: |
-          jupytext -k python3 --execute docs/tutorials/${{ matrix.tutorial }}
+          uvx hatch run docs:run_tutorial docs/tutorials/${{ matrix.tutorial }}
diff --git a/.github/workflows/test.yaml b/.github/workflows/test.yaml
index c4f9ad332..c0490c586 100644
--- a/.github/workflows/test.yaml
+++ b/.github/workflows/test.yaml
@@ -12,11 +12,6 @@ concurrency:
   group: ${{ github.workflow }}-${{ github.ref }}
   cancel-in-progress: true
 
-defaults:
-  run:
-    # to fail on error in multiline statements (-e), in pipes (-o pipefail), and on unset variables (-u).
-    shell: bash -euo pipefail {0}
-
 jobs:
   # Get the test environment from hatch as defined in pyproject.toml.
   # This ensures that the pyproject.toml is the single point of truth for test definitions and the same tests are
@@ -28,12 +23,12 @@ jobs:
     outputs:
       envs: ${{ steps.get-envs.outputs.envs }}
     steps:
-      - uses: actions/checkout@v4
+      - uses: actions/checkout@v5
         with:
           filter: blob:none
           fetch-depth: 0
       - name: Install uv
-        uses: astral-sh/setup-uv@v5
+        uses: astral-sh/setup-uv@v7
       - name: Get test environments
         id: get-envs
         run: |
@@ -51,6 +46,8 @@ jobs:
   # Run tests through hatch. Spawns a separate runner for each environment defined in the hatch matrix obtained above.
   test:
     needs: get-environments
+    permissions:
+       id-token: write  # for codecov OIDC
 
     strategy:
       fail-fast: false
@@ -63,15 +60,14 @@ jobs:
     continue-on-error: ${{ contains(matrix.env.name, 'pre') }}
 
     steps:
-      - uses: actions/checkout@v4
+      - uses: actions/checkout@v5
         with:
           filter: blob:none
           fetch-depth: 0
       - name: Install uv
-        uses: astral-sh/setup-uv@v5
+        uses: astral-sh/setup-uv@v7
         with:
           python-version: ${{ matrix.env.python }}
-          cache-dependency-glob: pyproject.toml
       - name: create hatch environment
         run: uvx hatch env create ${{ matrix.env.name }}
       - name: run tests using hatch
@@ -88,6 +84,9 @@ jobs:
           uvx hatch run ${{ matrix.env.name }}:coverage xml # create report for upload
       - name: Upload coverage
         uses: codecov/codecov-action@v5
+        with:
+          fail_ci_if_error: true
+          use_oidc: true
 
   # Check that all tests defined above pass. This makes it easy to set a single "required" test in branch
   # protection instead of having to update it frequently. See https://github.com/re-actors/alls-green#why.
diff --git a/.pre-commit-config.yaml b/.pre-commit-config.yaml
index f6b9fcd2a..27d8a954d 100644
--- a/.pre-commit-config.yaml
+++ b/.pre-commit-config.yaml
@@ -7,7 +7,7 @@ default_stages:
 minimum_pre_commit_version: 2.16.0
 repos:
   - repo: https://github.com/biomejs/pre-commit
-    rev: v2.3.8
+    rev: v2.3.10
     hooks:
       - id: biome-format
         exclude: ^\.cruft\.json$ # inconsistent indentation with cruft - file never to be modified manually.
@@ -16,7 +16,7 @@ repos:
     hooks:
       - id: pyproject-fmt
   - repo: https://github.com/astral-sh/ruff-pre-commit
-    rev: v0.14.9
+    rev: v0.14.10
     hooks:
       - id: ruff-check
         types_or: [python, pyi, jupyter]
diff --git a/.readthedocs.yaml b/.readthedocs.yaml
index c3f3f96fb..6c2847716 100644
--- a/.readthedocs.yaml
+++ b/.readthedocs.yaml
@@ -3,7 +3,8 @@ version: 2
 build:
   os: ubuntu-24.04
   tools:
-    python: "3.12"
+    python: "3.13"
+    nodejs: latest
   jobs:
     create_environment:
       - asdf plugin add uv
diff --git a/README.md b/README.md
index a4d4c0704..a503c6901 100644
--- a/README.md
+++ b/README.md
@@ -49,7 +49,7 @@ Please refer to the [documentation][link-docs]. In particular, the
 
 ## Installation
 
-You need to have Python 3.10 or newer installed on your system. If you don't have
+You need to have Python 3.11 or newer installed on your system. If you don't have
 Python installed, we recommend installing [Mambaforge](https://github.com/conda-forge/miniforge#mambaforge).
 
 There are several alternative options to install scirpy:
diff --git a/docs/conf.py b/docs/conf.py
index fddf73a84..75efdb9cc 100644
--- a/docs/conf.py
+++ b/docs/conf.py
@@ -5,11 +5,14 @@
 # https://www.sphinx-doc.org/en/master/usage/configuration.html
 
 # -- Path setup --------------------------------------------------------------
-import os
+import shutil
 import sys
 from datetime import datetime
 from importlib.metadata import metadata
 from pathlib import Path
+import os
+
+from sphinxcontrib import katex
 
 HERE = Path(__file__).parent
 sys.path.insert(0, str(HERE / "extensions"))
@@ -19,7 +22,7 @@
 # NOTE: If you installed your project in editable mode, this might be stale.
 #       If this is the case, reinstall it to refresh the metadata
 info = metadata("scirpy")
-project_name = info["Name"]
+project = info["Name"]
 author = info["Author"]
 copyright = f"{datetime.now():%Y}, {author}."
 version = info["Version"]
@@ -39,10 +42,10 @@
 
 html_context = {
     "display_github": True,  # Integrate GitHub
-    "github_user": "scverse",  # Username
-    "github_repo": project_name,  # Repo name
-    "github_version": "main",  # Version
-    "conf_py_path": "/docs/",  # Path in the checkout to the docs root
+    "github_user": "scverse",
+    "github_repo": project,
+    "github_version": "main",
+    "conf_py_path": "/docs/",
 }
 
 # Set canonical URL from the Read the Docs Domain
@@ -63,9 +66,9 @@
     "sphinx.ext.autosummary",
     "sphinx.ext.napoleon",
     "sphinxcontrib.bibtex",
+    "sphinxcontrib.katex",
     "sphinx_autodoc_typehints",
     "sphinx_tabs.tabs",
-    "sphinx.ext.mathjax",
     "IPython.sphinxext.ipython_console_highlighting",
     "sphinxext.opengraph",
     *[p.stem for p in (HERE / "extensions").glob("*.py")],
@@ -139,9 +142,9 @@
 #
 html_theme = "sphinx_book_theme"
 html_static_path = ["_static"]
-html_title = project_name
-html_logo = "img/scirpy_logo.png"
 html_css_files = ["css/custom.css"]
+html_title = project
+html_logo = "img/scirpy_logo.png"
 
 html_theme_options = {
     "repository_url": repository_url,
@@ -151,6 +154,7 @@
 }
 
 pygments_style = "default"
+katex_prerender = shutil.which(katex.NODEJS_BINARY) is not None
 
 # -- nbsphinx Tutorials ----------------------------------------------------------------
 
diff --git a/docs/contributing.md b/docs/contributing.md
index 699d94291..b172fd1d0 100644
--- a/docs/contributing.md
+++ b/docs/contributing.md
@@ -62,9 +62,9 @@ This will list “Standalone” environments and a table of “Matrix” environ
 +------------+---------+--------------------------+----------+---------------------------------+-------------+
 | Name       | Type    | Envs                     | Features | Dependencies                    | Scripts     |
 +------------+---------+--------------------------+----------+---------------------------------+-------------+
-| hatch-test | virtual | hatch-test.py3.10-stable | dev      | coverage-enable-subprocess==1.0 | cov-combine |
-|            |         | hatch-test.py3.13-stable | test     | coverage[toml]~=7.4             | cov-report  |
-|            |         | hatch-test.py3.13-pre    |          | pytest-mock~=3.12               | run         |
+| hatch-test | virtual | hatch-test.py3.11-stable | dev      | coverage-enable-subprocess==1.0 | cov-combine |
+|            |         | hatch-test.py3.14-stable | test     | coverage[toml]~=7.4             | cov-report  |
+|            |         | hatch-test.py3.14-pre    |          | pytest-mock~=3.12               | run         |
 |            |         |                          |          | pytest-randomly~=3.15           | run-cov     |
 |            |         |                          |          | pytest-rerunfailures~=14.0      |             |
 |            |         |                          |          | pytest-xdist[psutil]~=3.5       |             |
@@ -73,18 +73,18 @@ This will list “Standalone” environments and a table of “Matrix” environ
 ```
 
 From the `Envs` column, select the environment name you want to use for development.
-In this example, it would be `hatch-test.py3.13-stable`.
+In this example, it would be `hatch-test.py3.14-stable`.
 
 Next, create the environment with
 
 ```bash
-hatch env create hatch-test.py3.13-stable
+hatch env create hatch-test.py3.14-stable
 ```
 
 Then, obtain the path to the environment using
 
 ```bash
-hatch env find hatch-test.py3.13-stable
+hatch env find hatch-test.py3.14-stable
 ```
 
 In case you are using VScode, now open the command palette (Ctrl+Shift+P) and search for `Python: Select Interpreter`.
diff --git a/pyproject.toml b/pyproject.toml
index f835f0c01..294e99773 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -9,10 +9,9 @@ readme = "README.md"
 license = { file = "LICENSE" }
 maintainers = [ { name = "Gregor Sturm", email = "mail@gregor-sturm.de" } ]
 authors = [ { name = "Gregor Sturm" }, { name = "Tamas Szabo" } ]
-requires-python = ">=3.10"
+requires-python = ">=3.11"
 classifiers = [
   "Programming Language :: Python :: 3 :: Only",
-  "Programming Language :: Python :: 3.10",
   "Programming Language :: Python :: 3.11",
   "Programming Language :: Python :: 3.12",
   "Programming Language :: Python :: 3.13",
@@ -30,7 +29,7 @@ dependencies = [
   "logomaker!=0.8.5",
   "mudata>=0.2.3",
   "networkx>=2.5",
-  "numba>=0.57",
+  "numba>=0.60",
   "numpy>=1.17",
   "pandas>=1.5,!=2.1.2",
   "pooch>=1.7",
@@ -42,12 +41,31 @@ dependencies = [
   "squarify",
   "tqdm>=4.63",                           # https://github.com/tqdm/tqdm/issues/1082
 ]
-
 optional-dependencies.cupy = [ "cupy-cuda12x" ]
 optional-dependencies.dandelion = [ "sc-dandelion>=0.3.5" ]
-optional-dependencies.dev = [ "pre-commit" ]
 optional-dependencies.diversity = [ "scikit-bio>=0.5.7" ]
-optional-dependencies.doc = [
+optional-dependencies.parasail = [
+  # parasail 1.2.1 fails to be installd on MacOS
+  "parasail!=1.2.1",
+]
+optional-dependencies.rpack = [ "rectangle-packer" ]
+urls.Documentation = "https://scirpy.readthedocs.io/"
+urls.Home-page = "https://github.com/scverse/scirpy"
+urls.Source = "https://github.com/scverse/scirpy"
+
+[dependency-groups]
+dev = [ "pre-commit" ]
+test = [
+  "anndata>=0.9",
+  "black",
+  "coverage",
+  "parasail!=1.2.1",
+  "pytest",
+  "rectangle-packer",
+  # "sc-dandelion>=0.3.5",
+  "scikit-bio>=0.5.7",
+]
+doc = [
   "docutils>=0.8,!=0.18.*,!=0.19.*",
   # for tutorial
   "ipykernel",
@@ -69,53 +87,39 @@ optional-dependencies.doc = [
   "sphinx-copybutton",
   "sphinx-tabs",
   "sphinxcontrib-bibtex>=1",
+  "sphinxcontrib-katex",
   "sphinxext-opengraph",
-  # TODO needed for AnnData type hints
-  "zarr<3",
-]
-optional-dependencies.parasail = [
-  # parasail 1.2.1 fails to be installd on MacOS
-  "parasail!=1.2.1",
+  "zarr",                     # required for anndata typehints
 ]
-optional-dependencies.rpack = [ "rectangle-packer" ]
-optional-dependencies.test = [
-  "black",
-  "coverage",
-  "parasail!=1.2.1",
-  "pytest",
-  "rectangle-packer",
-  "sc-dandelion>=0.3.5",
-  "scikit-bio>=0.5.7",
-]
-urls.Documentation = "https://scirpy.readthedocs.io/"
-urls.Home-page = "https://github.com/scverse/scirpy"
-urls.Source = "https://github.com/scverse/scirpy"
 
 [tool.hatch.version]
 source = "vcs"
 
 [tool.hatch.envs.default]
 installer = "uv"
-features = [ "dev" ]
+dependency-groups = [ "dev" ]
 
 [tool.hatch.envs.docs]
-features = [ "doc" ]
+dependency-groups = [ "doc" ]
 scripts.build = "sphinx-build -M html docs docs/_build -W {args}"
 scripts.open = "python -m webbrowser -t docs/_build/html/index.html"
 scripts.clean = "git clean -fdX -- {args:docs}"
+scripts.run_tutorial = "jupytext -k python3 --execute {args}"
 
 # Test the lowest and highest supported Python versions with normal deps
 [[tool.hatch.envs.hatch-test.matrix]]
 deps = [ "stable" ]
-python = [ "3.10", "3.13" ]
+python = [ "3.11", "3.14" ]
 
 # Test the newest supported Python version also with pre-release deps
 [[tool.hatch.envs.hatch-test.matrix]]
 deps = [ "pre" ]
-python = [ "3.13" ]
+python = [ "3.14" ]
 
 [tool.hatch.envs.hatch-test]
-features = [ "dev", "test" ]
+dependency-groups = [ "dev", "test" ]
+default-args = [
+] # accounting for non-default tests path (specified via pytest.ini_options)
 
 [tool.hatch.envs.hatch-test.overrides]
 # If the matrix variable `deps` is set to "pre",
@@ -192,10 +196,14 @@ lint.per-file-ignores."src/scirpy/datasets/_processing_scripts/*" = [
 lint.per-file-ignores."tests/*" = [ "D" ]
 lint.pydocstyle.convention = "numpy"
 
-[tool.pytest.ini_options]
-testpaths = "src/scirpy/tests"
+[tool.pytest]
+strict = true
 xfail_strict = true
-addopts = [ "-m not gpu" ]
+addopts = [
+  "--import-mode=importlib", # allow using test files with same name
+  "-m not gpu",
+]
+testpaths = [ "src/scirpy/tests" ]
 markers = [
   "conda: marks a subset of tests to be ran on the Bioconda CI.",
   "extra: marks tests that require extra dependencies.",