diff --git a/.github/workflows/test.yml b/.github/workflows/test.yml
index f4dde8c..7734c9c 100644
--- a/.github/workflows/test.yml
+++ b/.github/workflows/test.yml
@@ -12,7 +12,7 @@ jobs:
     strategy:
       matrix:
         os: [ubuntu-latest]
-        python-version: ["3.9", "3.10", "3.11", "3.12"]
+        python-version: ["3.10", "3.11", "3.12", "3.13"]
         include:
           - os: macos-13
             python-version: "3.12"
diff --git a/README.rst b/README.rst
index c33e195..36099b5 100644
--- a/README.rst
+++ b/README.rst
@@ -85,7 +85,7 @@ News
 Requirements
 ============
 
-* `Python 3.9+ <https://www.python.org/downloads/release/python-390/>`_
+* `Python 3.10+ <https://www.python.org/downloads/release/python-3100/>`_
 * `Pip <https://pip.pypa.io>`_ (installed by default in newer versions of Python)
 * `Neuron 7.4+ <http://neuron.yale.edu/>`_ (compiled with Python support)
 * `eFEL eFeature Extraction Library <https://github.com/openbraininstitute/eFEL>`_ (automatically installed by pip)
diff --git a/pyproject.toml b/pyproject.toml
index 9a317df..dd0d8b9 100644
--- a/pyproject.toml
+++ b/pyproject.toml
@@ -10,7 +10,7 @@ authors = [
 description="Blue Brain Python Optimisation Library (bluepyopt)"
 readme = "README.rst"
 license = {file = "LICENSE.txt"}
-requires-python = ">= 3.9"
+requires-python = ">= 3.10"
 dynamic = ["version"]
 dependencies = [
     "numpy>=1.6",
diff --git a/tox.ini b/tox.ini
index 60af05f..fd730e7 100644
--- a/tox.ini
+++ b/tox.ini
@@ -4,14 +4,14 @@ minversion = 4
 
 [gh-actions]
 python =
-    3.9: py3
     3.10: py3
     3.11: py3
     3.12: py3
+    3.13: py3
 
 [testenv]
 envdir =
-    py3{9,10,11,12,}{-unit,-functional,-style,-syntax}: {toxworkdir}/py3
+    py3{10,11,12,13,}{-unit,-functional,-style,-syntax}: {toxworkdir}/py3
     docs: {toxworkdir}/docs
 extras = tests
 deps =
@@ -45,7 +45,7 @@ commands =
     functional: pytest --cov=bluepyopt {[testenv]coverage_options} bluepyopt/tests -m neuroml
 
 [testenv:docs]
-basepython = python3.9
+basepython = python3.12
 changedir = docs
 deps =
     sphinx