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我按照gitbook上面分享的脚本mapping small RNA seq数据,但是从sam 转换成bam文件的时候,提示有以下错误:
W::sam_hdr_parse] Duplicated sequence 'chr1:17368-17436'
[W::sam_hdr_parse] Duplicated sequence 'chr1:30365-30503'
[W::sam_hdr_parse] Duplicated sequence 'chr1:187890-187958'
[W::sam_hdr_parse] Duplicated sequence 'chr1:632324-632413'
[W::sam_hdr_parse] Duplicated sequence 'chr1:1167103-1167198'
请问这个是因为什么?对后续reads length 分布统计会有影响吗?
谢谢!
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