Calcium activation with calcium transient

[general-rishkin]

1. How would one go about adding calcium activation?
2. How would one go about using a pre-recorded calcium transient over a time period?

[cdw]

Hey hey,

Actin permissiveness is an analog for calcium activation. In setting this you are combining Tn/Tm interactions into a single variable. To use a pre-recorded Ca profile you'd set actin permissiveness to different values across the length of a run.

Take a look at the metafile creation which manages these things for you in a run, or set them manually by accessing individual instances of hs.thin[x].binding_sites[y].permissiveness.

[general-rishkin]

That is good to know. I will try this. If I have any problems, I will let you know. Thanks.
Great library too by the way.

[general-rishkin]

Hi
I have just had time to turn my attention back to this again.
I was able to set the permissiveness on the sites to an arbitrary value using hs.thin[x].binding_sites[y].permissiveness . So, I know I can use a sequence of values from the pre-recorded Ca profile across a run. (1) Would this be correct?

However, I am unsure how with a pre-recorded Ca profile I would set each of the following for the actin_permissiveness workloop using the metafile: cycle frequency, phase relative to longest length point, duration of on time, time from 10 to 90% influx level, and the half-life of the Ca2+ out-pumping.

Could you provide some explanation. Many thanks.