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Description
Hello,
thank you for your great package!
I am encountering a problem get dbs contexts from a granges list that was given to me.
I subsetted to dbs first
dbs_grl <- get_mut_type(gr_list, type = "dbs", predefined_dbs_mbs = TRUE)
Then I realized the Ref and Alt columns needed to be DNAStringSet:
gr_list_1 <- lapply(dbs_grl, function(gr) {
# Convert the REF column to DNAStringSet
mcols(gr)$ref <- DNAStringSet(mcols(gr)$ref)
if (is.character(mcols(gr)$alt)) {
mcols(gr)$alt <- DNAStringSet(mcols(gr)$alt)
} else if (is.list(mcols(gr)$alt)) {
mcols(gr)$alt <- DNAStringSetList(lapply(mcols(gr)$alt, DNAStringSet))
}
return(gr)
})
However, I am getting the error when calling get_dbs_context:
Error: There should not be any variants with multiple alternative alleles.
You can remove these by using the `get_mut_type` function.
Traceback:
1. .get_dbs_context_gr(vcf_list)
2. .check_no_indels(gr)
3. .check_no_indels_gr(gr)
4. .find_substitution(gr)
5. .check_no_multi_alts(gr)
6. .check_no_multi_alts_gr(gr)
7. stop(paste0("There should not be any variants with multiple alternative alleles.\n",
. "You can remove these by using the `get_mut_type` function."),
. call. = FALSE)
8. .handleSimpleError(function (cnd)
. {
. watcher$capture_plot_and_output()
. cnd <- sanitize_call(cnd)
. watcher$push(cnd)
. switch(on_error, continue = invokeRestart("eval_continue"),
. stop = invokeRestart("eval_stop"), error = invokeRestart("eval_error",
. cnd))
. }, "There should not be any variants with multiple alternative alleles.\nYou can remove these by using the `get_mut_type` function.",
. base::quote(NULL))
I looked at the source code for .check_no_multi_alts_gr(gr).
.check_no_multi_alts_gr <- function(gr) {
alt <- .get_alt(gr)
nr_alts <- alt %>%
unlist() %>%
length()
if (length(gr) != nr_alts) {
stop(paste0(
"There should not be any variants with multiple alternative alleles.\n",
"You can remove these by using the `get_mut_type` function."
), call. = FALSE)
}
invisible(gr)
}
I manually looked at a few samples and confirmed length(gr) == nr_alts. I am not sure why I am getting the error.
Thank you for your help.
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