diff --git a/genomeBrowserViz/README.md b/genomeBrowserViz/README.md new file mode 100644 index 0000000..9815869 --- /dev/null +++ b/genomeBrowserViz/README.md @@ -0,0 +1,13 @@ +## UCSC Genome Browser Visualizations +Track data hubs can be used to visualize genomics signal tracks using the UCSC Genome Browser (for more information, please see the [Raney et al., 2014](https://academic.oup.com/bioinformatics/article/30/7/1003/232409) publication. + +We developed a track data hub to visualize maxATAC-derived _in-silico_ ChIP-seq datasets in tandem with chromatin accessibility data. Data can be explored in multiple ways using this tool. + +### Visualization of Genomic Regions +After loading the track data hub (link [here](https://gb.research.cchmc.org/hub/group/memoryCD4/hub.txt)) in "My Data" > "Track Hubs" on the [UCSC Genome Browser website](https://genome.ucsc.edu/index.html), you can select specific genomic locations to visualize all subpopulation-resolved maxATAC predictions and chromatin accessibility information in that area. The image below shows the promoter and gene body of _IFNG_: + +### Identification of Specific TFBS +Right-clicking on the maxATAC prediction track (as demonstrated below) expands the composite track such that all TFs with binding sites in that region can be visualized. + +### Visualization of Specific Subpopulations +If you would like to visualize maxATAC predictions for a subset of subpopulations (e.g., the Th1 resting and active subpopulations), click on the... and select the desired subopoulations to visualize.