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Description
Read2Tree version: 2.0.1
It seems if read files name have whitespaces, they are either not correctly passed to mafft, or mafft itself is unhappy.
To reproduce: proceed with the test example given in the README. Then
cd tests
cp sample_1.fastq "sample_1 copy.fastq"
cp sample_2.fastq "sample_2 copy.fastq"
read2tree --tree --standalone_path marker_genes --reads sample_1\ copy.fastq sample_2\ copy.fastq --output_path ./output --dna_reference dna_ref.fa
...
d2tree-test/bin/mafft --auto --thread 1 --maxiterate 1000 --amino /tmp/tmp9vel2wo0
2025-11-12 15:22:14 INFO sample_1 copy: Alignment of 20 OGs took 5.6129608154296875.
--- Mapping of reads to reference sequences ---
Mapping reads to species: 0%| | 0/5 [00:00<?, ? species/s]2025-11-12 15:22:14 INFO sample_1 copy: --- Mapping of reads to MNELE reference species ---
Mapping reads to species: 0%| | 0/5 [00:00<?, ? species/s]
after which it stops with an error without providing any information.
> echo $?
1
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